Journal: Cell reports

Article Title: Neutral ceramidase-dependent regulation of macrophage metabolism directs intestinal immune homeostasis and controls enteric infection

doi: 10.1016/j.celrep.2022.110560

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: CD73 (154 Sm) , Fluidigm , Cat# 3154019B; RRID: AB_2813854.

Techniques: Recombinant, Western Blot, Protease Inhibitor, Enzyme-linked Immunosorbent Assay, SYBR Green Assay, Cell Culture, IP Phosphatase Assay, Software

Journal: Journal of Cachexia, Sarcopenia and Muscle

Article Title: A negative feedback loop between fibroadipogenic progenitors and muscle fibres involving endothelin promotes human muscle fibrosis

doi: 10.1002/jcsm.12974

Figure Lengend Snippet: Antibody panel used for CyTOF

Article Snippet: CD73 , 168Er , AD2 , Fluidigm , 3168015B.

Techniques:

Journal: Journal of Cachexia, Sarcopenia and Muscle

Article Title: A negative feedback loop between fibroadipogenic progenitors and muscle fibres involving endothelin promotes human muscle fibrosis

doi: 10.1002/jcsm.12974

Figure Lengend Snippet: Human FAPs isolated from fibrotic muscles differ from those isolated from nonfibrotic muscles. ( A ) Uniform manifold approximation and projection (UMAP) map showing the distributions of cells from M CT (yellow), FibM CT (orange), and FibM OP (red) muscle biopsies. For each condition, nonmyogenic cells were extracted, and data from three to four patients were concatenated. Each dot represents a single cell, and 300 000 cells were used to obtain the map. The 37 markers used for the analysis are listed in Table . ( B ) UMAP plots showing the expression patterns of PDGFRα, CD90, CD105, and CD73 in nonmyogenic cells from M CT , FibM CT , and FibM OP muscle biopsies. Cells are coloured according to the intensity of the marker shown. ( C ) Expression dot plot of selected markers from the CyTOF analysis of nonmyogenic cells from fibrotic and nonfibrotic muscles. Dots are coloured according to the average intensity with which the marker was expressed, and the size of each dot shows the percentage of nonmyogenic cells expressing each marker in each condition: M CT , FibM CT , and FibM OP . ( D ) Adipogenic and osteogenic differentiation of human FAPs isolated from fibrotic or nonfibrotic muscles. Left panel: Oil red O staining of FAPs from M CT , FibM CT , and FibM OP muscle biopsies in adipogenic differentiation medium. Right panel: Alizarin red staining of FAP cells from M CT , FibM CT , and FibM OP muscle biopsies in osteogenic differentiation medium.

Article Snippet: CD73 , 168Er , AD2 , Fluidigm , 3168015B.

Techniques: Isolation, Muscles, Expressing, Marker, Staining

Journal: Cell stem cell

Article Title: Smooth muscle cell reprogramming in aortic aneurysms

doi: 10.1016/j.stem.2020.02.013

Figure Lengend Snippet: (A) Representative IMC images stained for GFP (green), CD105 (red), CD73 (blue), CD90 (yellow), Sca-1 (magenta), and CD44 (cyan) in the overlaid format and individual channel images (N=6 mice/group). Scale bar: 500 μm. (B) PhenoGraph clustering of all cell phenotype visualized as a distinct color on the tSNE plot. (C) tSNE plot of high-dimension single cell data. (D) Heatmap visualizing the marker intensity for each Phenograph. (E) (left) Cluster 17 location in the tissue section using HistoCAT spatial clustering analysis. (right) A high-magnification of (Ea) left. (F) A cartoon showing confetti color distribution in bone nodule area and adjacent to the bone nodule area. (G) TGFβR2iSMC-Apoe mice VSMCs adjacent to the bone nodule area are mixed labeled with red fluorescent protein (RFP), yellow fluorescent protein (YFP), nuclear (n) green fluorescent protein (GFP), or membrane associated (m) cyan fluorescent protein (CFP). Scale bar: 10 μm. (H) TGFβR2iSMC-Apoe mice VSMCs in the bone nodule media areas are labeled with single color fluorescent protein after 4 months of high cholesterol high fat diet. Scale bar: 10 μm. (I) Bar chart showing the proportions of each of the Confetti colors in TGFβR2iSMC-Apoe mice VSMCs in media ascending aorta adjacent to the bone nodule area and in bone nodule area. N=10 for TGFβR2iSMC-Apoe mice. See also Figure S4, Figure S5, and Table S2B.

Article Snippet: Rat monoclonal anti-CD73-154Sm (clone TY/11.8) , Fluidigm , Cat# 3154019B, RRID:AB_2813854.

Techniques: Staining, Marker, Labeling

Journal: Cell stem cell

Article Title: Smooth muscle cell reprogramming in aortic aneurysms

doi: 10.1016/j.stem.2020.02.013

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rat monoclonal anti-CD73-154Sm (clone TY/11.8) , Fluidigm , Cat# 3154019B, RRID:AB_2813854.

Techniques: Recombinant, Multiplex Assay, Staining, In Situ, Marker, Expressing, Plasmid Preparation, shRNA, Software